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Image Search Results
Journal: Scientific Reports
Article Title: Validation of a potency assay for CD34 + cell based therapy
doi: 10.1038/s41598-025-14828-w
Figure Lengend Snippet: Quantification of VEGF in the supernatant of ProtheraCytes cultures as a potency assay. ( A ) ( C ) Concentration of VEGF in cell culture supernatants after 9 days of CD34 + cell expansion from four healthy donors and 16 AMI patients (Phase I/IIb clinical trial). ( B ) No significant difference observed when VEGF concentration was compared between AMI patients and healthy donors, but a significant difference was observed between AMI patients and StemFeed culture medium as negative control (t-test, p = 0.0021). ( D ) No significant difference (t-test, p = 0.0670) when VEGF concentrations in AMI patient and healthy donor supernatants quantified with the conventional method (Human VEGF QuantiGlo ELISA) were compared to those quantified with the ELLA system and its VEGF cartridge. These results have also been normalized to the number of CD34 + cells obtained after expansion and can be found in Supplemental Fig. 1.
Article Snippet: The development of the potency assay based on the quantification of VEGF secreted by CD34 + cells from AMI patients in cell culture supernatants collected after 9 days of cell expansion was initiated using the traditional ELISA luminescence method with the
Techniques: Potency Assay, Concentration Assay, Cell Culture, Negative Control, Enzyme-linked Immunosorbent Assay
Journal: bioRxiv
Article Title: Phospotyrosine proteomics reveals novel Zap70 and Itk pathway targets downstream of TCR and CAR in Jurkat T cells
doi: 10.1101/2025.11.03.686362
Figure Lengend Snippet: Interleukin-2 secretion, but not CD69 expression, is significantly reduced by Soq treatment. (A) Dotplot showing the percentage of CD69-positive cells measured by flow cytometry after 24 hours of soluble antibody stimulation. (B) Dotplot showing the concentration of IL-2 in the cell culture supernatant measured by ELISA after 24 hours of soluble antibody stimulation. (C-D) As in (A-B), except for APC-pMHC stimulation. (E-F) As in (A-B), except for Raji-co-culture stimulation of a second-generation CD19-CAR. Statistical analysis for flow cytometry and ELISA data was performed using a one-way ANOVA followed by the Holm-Sidak correction of Fisher’s LSD pairwise comparisons, if applicable, and is available in Supporting Tables 13-18.
Article Snippet: Interleuken-2 abundance was assessed using the
Techniques: Expressing, Flow Cytometry, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Co-Culture Assay